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KMID : 0364819870250030198
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Korean Journal of Microbiology
1987 Volume.25 No. 3 p.198 ~ p.204
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Properties of biparental clones formed by spheroplast fusion of Psedomonas putida
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Lee, Joo Shil/이주실
Lee, Young Won/Lee, Yung Nok/이영원/이영록
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Abstract
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1
Bipareatal clones and recombinant clones were obtained by spheroplast fusion of Pseudomonasputida KU218R-3 and P. putida KU428. Formation of the fusion product was the most effective when the Pseudomonas spheroplast mixture were treated with 40% plyethyleneglycol(PEG) 6000 for 10min at room temperature. The fusants which selected by indirect method were obtained at an average frequency of 10.8%. Most of the fasants were biparental cloaes(10.4%) and the recombinant clones were produced In low yield(0.42%). Fusants, at the frequency of 4% were obtained without PEG 6000, which shows that fusion Is not strictly dependent on PEG. The stability of fusants were examined. Most of the biparental clones were segregated to parental form and late recombinants were formed on further propagation of biparental clone but the recombinant clones were very stable.
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